Coding

Part:BBa_K2207031:Design

Designed by: Junming Qian   Group: iGEM17_ZJU-China   (2017-10-23)


PhlE efflux pump


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 512
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1106
    Illegal AgeI site found at 1172
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We cloned the phlE gene from Pseudomonadaceae.fluorescens via colony PCR, and inserted it to pSB1C3 after being standardized.

Source

We cloned the phlE via colony PCR from Pseudomonas fluorescens 2P24, which is a gift from Prof. Liqun Zhang of China Agricultural University.

References

Yang F, Cao Y. Biosynthesis of phloroglucinol compounds in microorganisms—review[J]. Applied Microbiology & Biotechnology, 2012, 93(2):487-95.